Isolation and Identification of Helicase from Anoxybacillus sp for DNA amplification
نویسندگان
چکیده
منابع مشابه
Anoxybacillus ayderensis sp. nov. and Anoxybacillus kestanbolensis sp. nov.
Two thermophilic bacilli were isolated from mud and water samples of the Ayder and Kestanbol hot springs in the provinces of Rize and Canakkale, respectively, in Turkey. Strains AB04T and K4T were sporulating, Gram-positive, rod-shaped bacteria. These isolates were moderately thermophilic (with an optimum temperature for growth of 50-55 degrees C), facultative anaerobes able to grow on a wide r...
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Polymerase chain reaction is the most widely used method for in vitro DNA amplification. However, it requires thermocycling to separate two DNA strands. In vivo, DNA is replicated by DNA polymerases with various accessory proteins, including a DNA helicase that acts to separate duplex DNA. We have devised a new in vitro isothermal DNA amplification method by mimicking this in vivo mechanism. He...
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30 milled rice samples were collected from retailers in four states of Malaysia. These samples were evaluated for Eurotium spp. contaminations by direct plating on malt extract salt agar (MESA). All Eurotium were isolated and identified based on morphology and nucleotide sequences of internal transcribed spacer 1 (ITS1) and ITS2 of the rDNA. Four Eurotium species (E. rubrum, E. amstelodami, E....
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ژورنال
عنوان ژورنال: APCBEE Procedia
سال: 2012
ISSN: 2212-6708
DOI: 10.1016/j.apcbee.2012.06.029